Company portrait
EXBIO is the leading manufacturer of monoclonal antibodies and reagents for research and clinical applications with a focus on multicoloured flow cytometry analysis of human cell markers. Our broad portfolio of over 4000 ready-to-use products covers a diverse set of areas including haematology. , immunology, cancer, stem cells and cell biology. With experts in cytometry and biotechnology engineers, EXBIO offers our customers long-term validated antibody markers in the wide selection of fluorochrome conjugates.
Our commitment to customer needs, backed by our integrated quality management system that complies with ISO 13485: 2016, ISO9001: 2015 and cGMP (21 CFR part 820) standards, enables us to fulfil our mission:
EXBIO strives to exceed the expectations of the most demanding clients in the field of analytical cytometry by providing a complete portfolio of high-quality products and services at affordable prices.
Manufacturer of reagents and mAbs for research and clinical applications
EXBIO is the leading manufacturer of monoclonal antibodies and reagents for research and clinical applications with a focus on multi-colour flow cytometric analysis of human cell markers. Its comprehensive portfolio of more than 4,000 ready-to-use products covers a diverse set of areas including haematology, immunology, cancer, stem cells, and cell biology.
With experts in cytometry and biotechnology engineers, EXBIO offers long-term validated antibody markers in the wide selection of fluorochrome conjugates. Bio Connect Diagnostics supplies all of its products in Benelux. If you have any questions, contact Technical Support.
EXBIO’s main product lines
- Antibodies
- Protein
- Test kits
- Diagnostic Products
METHODS
Complete blood count, manual and digital cell differential
A complete blood count (CBC) was performed on a Sysmex XE 5000 Hematology Analyzer (Kobe, Japan). The Sysmex SP1000i Automatic Staining Machine / Stainer was used to slide smear preparation and peripheral blood staining
(PB) samples. At least two independent morphologists evaluated 200 cells per slide to provide a cell differential. Sysmex Cellavision DM8 produced digital cell differential after analysis of 100 cells per slide.
Immunophenotyping by flow cytometry
Immunophenotyping was performed by flow cytometry. on a Beckman Coulter FC500 five-colour flow cytometer (BC International; Nyon, Switzerland). Fluorescence tagging Antibodies for staining of samples were obtained from Beckman Coulter, EXBIO (Praha, Czech Republic), Dako. (Glostrup, Denmark) and Becton Dickinson (BD; San José, CALIFORNIA).
The samples were prepared with a whole blood lysis enumeration method and phenotypic surface characterization of PCs, as recommended. Fixation and
permeabilization was also used for the analysis of the expression of intracellular light chains.
Briefly, 50 aliquots of blood samples were incubated with the conjugated antibodies as suggested by the manufacturers and lysed with an ammonium chloride-based lysis solution (BC International; Nyon, Switzerland). For intracellular staining, 50 ll aliquots of blood samples were incubated
with the conjugated antibodies for surface staining, subsequently fixed and permeabilized with the FIX & PERM kit (Invitrogen; Carlsberg, CA), and then incubated with the Conjugated antibodies for intracellular staining.